Hebei New Century Pharmaceutical Co., Ltd.

Name: High-performance liquid chromatography
Description: High-performance liquid chromatography (or High pressure liquid chromatography, HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry to separate, identify, and quantify compounds. HPLC utilizes a column that holds chromatographic packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that shows the retention times of the molecules. Retention time varies depending on the interactions between the stationary phase, the molecules being analyzed, and the solvent(s) used. Note: It is important to read the introductory page about thin layer chromatography before you continue with this one - particularly the part about how thin layer chromatography works. High performance liquid chromatography works on the same basic principle. HPLC is essentially an adaptation of column chromatography - so it might be a good idea to have a (very quick) look at that as well.